"Cassures double-brin de l'ADN -- Dissertation universitaire" . . . "Spectrom\u00E9trie de masse" . "Antig\u00E8ne nucl\u00E9aire de prolif\u00E9ration cellulaire -- Dissertation universitaire" . . . "Prot\u00E9omique" . . "2007" . . "Fuselloviridae -- Dissertation universitaire" . "Text" . . . "Arch\u00E9obact\u00E9ries -- Effets du rayonnement ultraviolet" . . . . "Identification of proteins involved in DNA repair in Sulfolobus solfataricus" . . . "S. solfataricus is a thermoacidophilic Archaea that lives at 80\u00B0C where the frequency of DNA damage is strongly increased. Therefore this organism must have an efficient repair system. S. solfataricus response to gamma- and UV-rays was studied by using a comparative proteomic approach. We showed that S. solfataricus is resistant to doses up to 1000 Gray although this organism has no particular protection system against DNA double strand breaks. The kinetic of DNA repair reveals that this archaeon has a very efficient repair system. At a proteomic level, we identified 17 protein presenting variations after irradiation, among which replication proteins PCNA-2 and Cdc6-2. To validate the effects of UV rays, we used the temperate virus temperate SSV1 which can infect in a stable way our model without affecting its growth or its proteome as a good. After irradiation of both isogenetic strains, we observed a reduced growth rate that was more pronounced in infected cells. Only the infected strain presented significant variations of its proteome. So, the presence of the virus amplified the signal of cell response. By mass spectrometry, we identified 23 proteins among which proteins implicated in oxidative stress response and DNA metabolism. Hence, our work reveals that regardless of the nature of DNA damage in S. solfataricus, the proteome undergoes few variations. In agreement with literature, this suggest that S. solfataricus recruits repair proteins to the damage site by post-translational modifications with most probably a relocalization in the cell for a faster and more efficient repair of damage at high temperatures" . "Identification des prot\u00E9ines impliqu\u00E9es dans la r\u00E9paration de l'ADN chez Sulfolobus solfataricus" . "Identification des prot\u00E9ines impliqu\u00E9es dans la r\u00E9paration de l'ADN chez Sulfolobus solfataricus" . . "Th\u00E8ses et \u00E9crits acad\u00E9miques" . "Prot\u00E9ome -- Dissertation universitaire" . . "Sulfolobus solfataricus -- Dissertation universitaire" . . . . . . "S. solfataricus est un Archaea thermoacidophile vivant \u00E0 80\u00B0C, temp\u00E9rature o\u00F9 la fr\u00E9quence des dommages de l'ADN est fortement augment\u00E9e ce qui suppose qu\u2019il poss\u00E8de un syst\u00E8me de r\u00E9paration de son g\u00E9nome tr\u00E8s efficace. La r\u00E9ponse cellulaire de S. solfataricus aux rayonnements gamma et aux UV a \u00E9t\u00E9 suivie par une approche de prot\u00E9omique diff\u00E9rentielle. Nous avons montr\u00E9 que S. solfataricus r\u00E9siste \u00E0 des doses allant jusqu'\u00E0 1000 Gray alors qu\u2019il ne poss\u00E8de aucun syst\u00E8me de protection des cassures double-brin de son ADN. La cin\u00E9tique de r\u00E9paration de l\u2019ADN indique qu\u2019il dispose d\u2019un syst\u00E8me de r\u00E9paration efficace. Dans son prot\u00E9ome, nous avons identifi\u00E9 par spectrom\u00E9trie de masse 17 prot\u00E9ines pr\u00E9sentant des variations apr\u00E8s irradiation, dont les prot\u00E9ines de r\u00E9plication PCNA-2 et Cdc6-2. Enfin, par marquage sp\u00E9cifique des prot\u00E9ines, nous avons visualis\u00E9 une variation importante de la dynamique des prot\u00E9ines de cellules irradi\u00E9es. Pour visualiser clairement la r\u00E9ponse de S. solfataricus, nous avons utilis\u00E9 une souche infect\u00E9e par le virus temp\u00E9r\u00E9 SSV1. La comparaison des prot\u00E9omes nous a permis d\u2019identifier 23 prot\u00E9ines par spectrom\u00E9trie de masse, dont une partie intervenant dans le stress oxydant et le m\u00E9tabolisme de l\u2019ADN. Ainsi, nos travaux montrent que, quelle que soit la nature des dommages subis par S. solfataricus, son prot\u00E9ome subit tr\u00E8s peu de variations. Les prot\u00E9ines de r\u00E9paration pourraient \u00EAtre recrut\u00E9es aux sites de l\u00E9sions par modifications post-traductionnelles et/ou par relocalisation dans la cellule pour une r\u00E9paration rapide et efficace des l\u00E9sions de l\u2019ADN" .