. "Endocytose" . "Candida albicans (C. albicans), a yeast belonging to the human microbiota, can cause of severe and potentially fatal systemic infections in debilitated patients. The gastrointestinal tract is recognized as the main source of disseminated C. albicans infections, the enterocyte barrier playing a protective role against these infections. The tight junctions (TJs) connecting adjacent enterocytes provide impermeability and integrity of the intestinal barrier. The research work of this thesis concerns the modulation of the integrity of TJs during the infection of enterocytes by C. albicans and the specification of the molecular and cellular mechanism allowing the fungus to cross the intestinal barrier. A model of interaction between the intestinal barrier and C. albicans has been developed. In this context, tissue permeability is increased upon treatment of the Caco-2 cell monolayers with the secretome of C. albicans SC5314. The disruption of the epithelial barrier is correlated with a modulation of the functionnality of the enterocyte TJs and involves a fungal peptide belonging to the protein Repressed by tup1 (Rbt1). Consequently, the translocation of C. albicans from the intestinal flora to the underlying tissues is potentiated, favoring its tissue invasion by endocytosis mechanism." . . "2021" . . . "Ent\u00E9rocytes" . . "Les jonctions serr\u00E9es des ent\u00E9rocytes comme cible pour la translocation de Candida albicans \u00E0 travers la barri\u00E8re intestinale" . . . . "Les jonctions serr\u00E9es des ent\u00E9rocytes comme cible pour la translocation de Candida albicans \u00E0 travers la barri\u00E8re intestinale" . . "Enterocyte tight junctions as a target for Candida albicans translocation through the gut barrier." . . . . "Candida albicans (C. albicans) est une levure appartenant au microbiote de l\u2019Homme, pouvant \u00EAtre \u00E0 l\u2019origine d\u2019infections syst\u00E9miques s\u00E9v\u00E8res et potentiellement mortelles chez les patients fragilis\u00E9es. Le tractus gastro-intestinal est reconnu comme la principale source d\u2019infections diss\u00E9min\u00E9es \u00E0 C. albicans, la barri\u00E8re ent\u00E9rocytaire jouant un r\u00F4le protecteur vis-\u00E0-vis de ces infections. Les jonctions serr\u00E9es (JSs) reliant les ent\u00E9rocytes adjacents assurent l\u2019imperm\u00E9abilit\u00E9 et l\u2019int\u00E9grit\u00E9 de la barri\u00E8re intestinale. Les travaux de recherche de cette th\u00E8se portent sur l\u2019\u00E9tude de la modulation de l\u2019int\u00E9grit\u00E9 des JSs au cours de l\u2019infection des ent\u00E9rocytes par C. albicans et la sp\u00E9cification des m\u00E9canismes cellulaire et mol\u00E9culaire permettant au champignon de traverser la barri\u00E8re intestinale. Un mod\u00E8le d\u2019interaction entre la barri\u00E8re digestive et C. albicans a \u00E9t\u00E9 d\u00E9velopp\u00E9. Dans ce contexte, la perm\u00E9abilit\u00E9 tissulaire est augment\u00E9e lors du traitement de la monocouche de cellules Caco-2 avec du s\u00E9cr\u00E9tome de C. albicans SC5314. La rupture de l\u2019int\u00E9grit\u00E9 de la barri\u00E8re \u00E9pith\u00E9liale est corr\u00E9l\u00E9e avec une modulation de la fonctionnalit\u00E9 des JSs ent\u00E9rocytaires et implique un peptide fongique appartenant \u00E0 la prot\u00E9ine Repressed by tup1 (Rbt1). En cons\u00E9quence, la translocation de C. albicans de la flore intestinale vers les tissus sous-jacents est potentialis\u00E9e, favorisant son invasion tissulaire par m\u00E9canisme d\u2019endocytose." . "Candida albicans" . . . . "Ent\u00E9rocytes Caco-2" . . . "Text" . "Invasion fongique" . "Jonctions serr\u00E9es" . . "Th\u00E8ses et \u00E9crits acad\u00E9miques" .